Zika-only virus detection (CDC Trioplex), RT-PCR

The Trioplex Real-time RT-PCR Assay (Trioplex rRT-PCR) is intended for the qualitative detection of Zika virus RNA in urine, and amniotic fluid (each collected alongside a patient-matched serum specimen). For use in individuals who meet the Centers for Disease Control and Prevention (CDC) clinical and epidemiological criteria for Zika virus testing. Zika viruses are primarily transmitted by Aedes aegypti and Aedes albopictus mosquitos.

Portal Ordering Code: TRIO-PCR-URINE/AMNIO
CPT Code(s): 87662, 87798x2

Fee Schedule

Storage and Transport Criteria: Specimen Storage Specimen Transport

Acceptable Specimens

Minimum Volume

Frozen Range
-70°C - 0°C

Refrigerated Range
0.1°C - 8°C

Room Temperature Range
8.1°C - 30°C

Stability at
4°C - 25°C

Urine or amniotic fluid 1 mL ≤ -20°C upon receipt Reject Reject Dry ice or cold packs (acceptable)
Includes: Serum order choice is automatically ordered
Methodology: RT-PCR
Assay Category: EUA (Emergency Use Authorization)
Reference Range: See laboratory report
Alternative Names: Trioplex Real-time RT-PCR Assay

ZIKA virus
Preferred Specimen(s): Urine

Amniotic fluid
Alternative Specimen(s): N/A
Minimum Volume: 1 mL
Special Instructions: Collect serum in conjunction with urine or amniotic fluid.
Reject Criteria: Inadequately labeled specimens

Leaking specimens

QNS (quantities not sufficient)

Specimens not meeting the Storage & Transport criteria upon receipt at laboratory
Setup Schedule: Testing is performed on an as-needed basis.
Turn around time (TAT): 2 business days from arrival at the lab

Acceptable Specimens	Minimum Volume	Frozen Range -70°C - 0°C	Refrigerated Range 0.1°C - 8°C	Room Temperature Range 8.1°C - 30°C	Stability at 4°C - 25°C
		Specimen Storage			Specimen Transport
Urine or amniotic fluid	1 mL	≤ -20°C upon receipt	Reject	Reject	Dry ice or cold packs (acceptable)