Shiga Toxin - Positive fecal broths (Title 17)

A multiplex real-time PCR assay detecting the presence of DNA from Shiga toxin producing Escherichia coli. Assay specifically detects two Shiga Toxin encoding genes, stx1 and stx2, and the dppD gene which is specific to Escherichia coli.

Portal Ordering Code: STX broth
CPT Code(s): 87150x2

Fee Schedule

Storage and Transport Criteria: Specimen Storage Specimen Transport

Acceptable Specimens

Minimum Volume

Frozen Range
-70°C - 0°C

Refrigerated Range
0.1°C - 8°C

Room Temperature Range
8.1°C - 30°C

Stability at
4°C - 25°C

Stool in GN broth or selenite broth 1 mL Reject Acceptable Acceptable Store and ship broths at 2°C - 8°C
Includes: Shiga Toxin-producing E. coli (STEC) by multiplex Real-Time PCR.
Methodology: Culture/PCR
Assay Category: LDT (Laboratory Developed Test)
Reference Range: see laboratory report
Alternative Names: Title 17
Preferred Specimen(s): Stool in GN broth

Stool selenite broth
Alternative Specimen(s): N/A
Minimum Volume: 1 mL
Special Instructions: N/A
Reject Criteria: Inadequately labeled specimens

Leaking specimens

QNS (quantities not sufficient)

Specimens not meeting the Storage & Transport criteria upon receipt at laboratory
Setup Schedule: Testing is performed on an as-needed basis
Turn around time (TAT): 4 weeks from arrival at the lab

Acceptable Specimens	Minimum Volume	Frozen Range -70°C - 0°C	Refrigerated Range 0.1°C - 8°C	Room Temperature Range 8.1°C - 30°C	Stability at 4°C - 25°C
		Specimen Storage			Specimen Transport
Stool in GN broth or selenite broth	1 mL	Reject	Acceptable	Acceptable	Store and ship broths at 2°C - 8°C