Syphilis – TPPA

The TP-PA test is designed to be used as an aid in the confirmation of antibodies to the treponemal organisms that cause syphilis. Other diseases such as yaws or pinta give positive results.

Portal Ordering Code: Syphilis-TPPA
CPT Code(s): 86780

Fee Schedule

Storage and Transport Criteria: Specimen Storage Specimen Transport

Acceptable Specimens

Minimum Volume

Frozen Range
-70°C - 0°C

Refrigerated Range
0.1°C - 8°C

Room Temperature Range
8.1°C - 30°C

Stability at
4°C - 25°C

Serum 1 mL Acceptable 1X frozen/thawed Acceptable up to 5 days Reject 8 hours

EDTA plasma, Sodium Citrate plasma, Heparin plasma 1 mL Reject Acceptable up to 48 hours Reject 8 hours
Includes: N/A
Methodology: Particle Agglutination
Assay Category: FDA Approved
Reference Range: See laboratory report
Alternative Names: T. pallidum Ab

TP-PA

Agglutination (TP-PA) Test

Syphilis
Preferred Specimen(s): Serum

EDTA plasma

Sodium Citrate plasma

Heparin plasma
Alternative Specimen(s): N/A
Minimum Volume: 1 mL
Special Instructions: Screening VDRL cannot be performed on plasma.
Reject Criteria: Inadequately labeled specimens

Leaking specimens

QNS (quantities not sufficient)

Specimens not meeting the Storage & Transport criteria upon receipt at laboratory
Setup Schedule: Testing done once a week.
Turn around time (TAT): 7 business days from arrival at the lab

Acceptable Specimens	Minimum Volume	Frozen Range -70°C - 0°C	Refrigerated Range 0.1°C - 8°C	Room Temperature Range 8.1°C - 30°C	Stability at 4°C - 25°C
		Specimen Storage			Specimen Transport
Serum	1 mL	Acceptable 1X frozen/thawed	Acceptable up to 5 days	Reject	8 hours
EDTA plasma, Sodium Citrate plasma, Heparin plasma	1 mL	Reject	Acceptable up to 48 hours	Reject	8 hours