Enterovirus screen, (includes EV-D68), RT-PCR
A screening assay to detect the presence of all enterovirus types including EV-D68 and poliovirus. EV-D68 is the causative agent for Acute flaccid myelitis (AFM) virus. EV is the causative agent for hand, foot, and mouth disease virus.
- Portal Ordering Code
ENTV PCR
- CPT Code(s)
87798
- Storage and Transport Criteria
-
Specimen Storage Specimen Transport Acceptable SpecimensMinimum VolumeFrozen Range
-70°C - 0°CRefrigerated Range
0.1°C - 8°CRoom Temperature Range
8.1°C - 30°CStability at
4°C - 25°CNasal or pharyngeal swabs, aspirates, throat swab, bronchial alveolar lavage, tracheal aspirate 2-3 mLs in VTM Acceptable - freeze at -20°C Acceptable at 2°C – 8°C up to 72 hours Reject 8 hours CSF 0.5 - 2 mLs Acceptable - freeze at -20°C Acceptable at 2°C – 8°C up to 72 hours Reject 8 hours - Includes
Positive specimens are forwarded to CDPH for further characterization.
- Methodology
RT-PCR
- Assay Category
LDT (Laboratory Developed Test)
- Reference Range
See laboratory report
- Alternative Names
- Coxsackie Virus PCR
- Echovirus PCR
- Preferred Specimen(s)
Nasal or pharyngeal swabs
- Alternative Specimen(s)
- CSF
- Aspirates
- Throat swab
- Bronchial alveolar lavage
- Tracheal aspirate
- Minimum Volume
- CSF - 0.5-2 mL
- Non-CSF - 2-3 mLs in VTM
- Special Instructions
N/A
- Reject Criteria
- Inadequately labeled specimens
- Leaking specimens
- QNS (quantities not sufficient)
- Cotton or calcium alginate swabs
- Swabs in Amies medium
- Swabs with wooden shafts
- Specimens not meeting the Storage & Transport criteria upon receipt at laboratory
- Setup Schedule
Testing is performed on an as-needed basis.
- Turn around time (TAT)
2 business days from arrival at the lab
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