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Department of Health Services

Public Health Regional Laboratory

MALDITOF Identification-Mycobacterial

Identification of mycobacteria using Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry is the primary identification method.

Portal Ordering Code

MALDITOF

CPT Code(s)

87118

Fee Schedule

Storage and Transport Criteria
Specimen Storage Specimen Transport
Acceptable Specimens
Minimum Volume
Frozen Range
-70°C - 0°C
Refrigerated Range
0.1°C - 8°C
Room Temperature Range
8.1°C - 30°C
Stability at
4°C - 25°C
Pure culture isolate None; Viable growth should be present on the submitted subculture Reject Acceptable Acceptable 8 hours
Includes

Ancillary testing (ie, 16S gene sequencing, conventional biochemicals) will be employed as required.  Additional fees may apply.

Methodology

Mass Spectrometry

Assay Category

LDT (Laboratory Developed Test)

Reference Range

See laboratory report

Alternative Names

MALDI

Preferred Specimen(s)
  • Pure culture isolate in solid media (Lowenstein-Jensen or Middlebrook 7H11)
  • Pure culture isolate in liquid media (BD BACTEC MGIT/modified Middlebrook 7H9)
Alternative Specimen(s)

N/A

Minimum Volume

None; Viable growth should be present on the submitted subculture.

Special Instructions

N/A

Reject Criteria
  • Inadequately labeled specimens
  • Leaking specimens
  • QNS (quantities not sufficient)
  • Specimens not meeting the Storage & Transport criteria upon receipt at laboratory
Setup Schedule

Testing is performed on an as-needed basis.

Turn around time (TAT)

4 weeks from arrival at the lab