Bacteriology Isolate Identification (Title 17)

For Title 17 - fee applies to Local Health Jurisdiction of submitting facility.

Identification of bacteria using selective media, biochemical tests (API-20E), MALDI-TOF, PCR, and antisera agglutination testing.

Storage and Transport Criteria: Specimen Storage Specimen Transport

Acceptable Specimens

Minimum Volume

Frozen Range
-70°C - 0°C

Refrigerated Range
0.1°C - 8°C

Room Temperature Range
8.1°C - 30°C

Stability at
4°C - 25°C

Bacteria isolate None; Viable growth should be present on the submitted subculture. Reject Acceptable Acceptable < 1 week
Includes: Further characterization if applicable
Methodology: Culture
Assay Category: FDA Approved
Reference Range: See laboratory report
Alternative Names: Title 17
Preferred Specimen(s): Isolate on nutrient agar:

Gram negative bacteria on Columbia Blood Agar with 5% Sheep Blood, Trypticase soy agar, MacConkey Agar

Gram positive bacteria on Trypticase soy agar with 5% sheep blood, Chocolate agar, Columbia CNA agar with 5% Sheep Blood
Alternative Specimen(s): N/A
Minimum Volume: None; Viable growth should be present on the submitted subculture.
Special Instructions: For Campylobacter species use Campylobacter Agar
Reject Criteria: Inadequately labeled specimens

Leaking specimens

QNS (quantities not sufficient)

Specimens not meeting the Storage & Transport criteria upon receipt at laboratory
Setup Schedule: Testing is performed on an as-needed basis.
Turn around time (TAT): 4 weeks from arrival at the lab

Acceptable Specimens	Minimum Volume	Frozen Range -70°C - 0°C	Refrigerated Range 0.1°C - 8°C	Room Temperature Range 8.1°C - 30°C	Stability at 4°C - 25°C
		Specimen Storage			Specimen Transport
Bacteria isolate	None; Viable growth should be present on the submitted subculture.	Reject	Acceptable	Acceptable	< 1 week