Zika, Dengue, & Chikungunya virus detection (CDC Trioplex), RT-PCR
The Trioplex Real-time RT-PCR Assay (Trioplex rRT-PCR) is intended for the qualitative detection and differentiation of RNA from Zika virus, dengue virus, and chikungunya virus in human sera or cerebrospinal fluid (each collected alongside a patientmatched serum specimen), and for the qualitative detection of Zika virus RNA in urine, and amniotic fluid (each collected alongside a patient-matched serum specimen). For use in individuals who meet the Centers for Disease Control and Prevention (CDC) clinical and epidemiological criteria for Zika virus testing. Zika viruses are primarily transmitted by Aedes aegypti and Aedes albopictus mosquitos.
- Portal Ordering Code
- TRIO-PCR-SERUM
- TRIO-PCR-CSF
- CPT Code(s)
87662, 87798x2
- Storage and Transport Criteria
-
Specimen Storage Specimen Transport Acceptable SpecimensMinimum VolumeFrozen Range
-70°C - 0°CRefrigerated Range
0.1°C - 8°CRoom Temperature Range
8.1°C - 30°CStability at
4°C - 25°CSerum or CSF 1 mL ≤ -20°C upon receipt Reject Reject Dry ice or cold packs (acceptable) - Includes
CSF - Serum order choice is automatically ordered.
- Methodology
RT-PCR
- Assay Category
EUA (Emergency Use Authorization)
- Reference Range
See laboratory report
- Alternative Names
- Trioplex Real-time RT-PCR Assay
- Chikungunya virus
- Dengue virus
- ZIKA virus
- Preferred Specimen(s)
- Serum
- CSF
- Alternative Specimen(s)
N/A
- Minimum Volume
1 mL
- Special Instructions
CSF - Collect serum in conjunction with CSF
- Reject Criteria
- Inadequately labeled specimens
- Leaking specimens
- QNS (quantities not sufficient)
- Specimens not meeting the Storage & Transport criteria upon receipt at laboratory
- Setup Schedule
Testing is performed on an as-needed basis.
- Turn around time (TAT)
3 business days from arrival at the lab
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