Brucella RT-PCR

This Real Time PCR assay detects Brucella species (including B. abortus, B. canis, B. melitensis, B. suis)

Storage and Transport Criteria: Specimen Storage Specimen Transport

Acceptable Specimens

Minimum Volume

Frozen Range
-70°C - 0°C

Refrigerated Range
0.1°C - 8°C

Room Temperature Range
8.1°C - 30°C

Stability at
4°C - 25°C

Isolate in nutrient agar N/A Reject Reject Acceptable 8 hours
Includes: Lysate forwarded to CDPH for species determination
Methodology: RT-PCR
Assay Category: LRN-Developed assay
Reference Range: See laboratory report
Alternative Names: Brucella Real Time PCR

B. abortus, B. canis, B. melitensis, B. suis

Brucellosis

Undulating fever
Preferred Specimen(s): Isolate in nutrient agar
Alternative Specimen(s): N/A
Minimum Volume: N/A
Special Instructions: N/A
Reject Criteria: Inadequately labeled specimens

Leaking specimens

QNS (quantities not sufficient)

Specimens not meeting the Storage & Transport criteria upon receipt at laboratory
Setup Schedule: Testing is performed on an as-needed basis.
Turn around time (TAT): 2 business days from arrival at the lab

Acceptable Specimens	Minimum Volume	Frozen Range -70°C - 0°C	Refrigerated Range 0.1°C - 8°C	Room Temperature Range 8.1°C - 30°C	Stability at 4°C - 25°C
		Specimen Storage			Specimen Transport
Isolate in nutrient agar	N/A	Reject	Reject	Acceptable	8 hours