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Department of Health Services

Public Health Regional Laboratory

Ehrlichia chaffeensis (HME) Immunofluorescent Assay (IFA)

Human Monocytic Ehrlichiosis (HME) is a tick-borne infection caused by Ehrlichia chaffeensis. Infections range in severity from asymptomatic to life-threatening, especially in patients who are immunocompromised.

Portal Ordering Code

EC IFA

CPT Code(s)

86666

Fee Schedule

Storage and Transport Criteria
Specimen Storage Specimen Transport
Acceptable Specimens
Minimum Volume
Frozen Range
-70°C - 0°C
Refrigerated Range
0.1°C - 8°C
Room Temperature Range
8.1°C - 30°C
Stability at
4°C - 25°C
Serum 1 mL Acceptable Acceptable Acceptable 8 hours
Includes

Positives titered to end point

Methodology

IFA

Assay Category

FDA Approved

Reference Range

See laboratory report

Alternative Names

Human Monocytic Ehrlichiosis

Preferred Specimen(s)

Serum

Alternative Specimen(s)

N/A

Minimum Volume

1 mL

Special Instructions

1 mL of serum is sufficient volume to perform all tick-borne disease serology assays. Submission of multiple tubes of serum in not necessary.

Reject Criteria
  • Inadequately labeled specimens
  • Leaking specimens
  • QNS (quantities not sufficient)
  • Specimens not meeting the Storage & Transport criteria upon receipt at laboratory
Setup Schedule

Testing done once a week.

Turn around time (TAT)

7 business days from arrival at the lab