Mycobacterium tuberculosis complex, RT-PCR with Rifampin, sputum (GeneXpert)

Detection and identification of Mycobacterium tuberculosis complex from sputum specimens. Test includes detection of an rpoB gene associated with rifampin resistance.

Portal Ordering Code: MTBRIF
CPT Code(s): 87556

Fee Schedule

Storage and Transport Criteria: Specimen Storage Specimen Transport

Acceptable Specimens

Minimum Volume

Frozen Range
-70°C - 0°C

Refrigerated Range
0.1°C - 8°C

Room Temperature Range
8.1°C - 30°C

Stability at
4°C - 25°C

Unprocessed sputum 1 mL Reject Acceptable up to 7 days Acceptable up to 35°C for 3 days 8 hours

Processed sediment 0.5 mL Reject Acceptable up to 7 days Reject 8 hours
Includes: N/A
Methodology: NAAT
Assay Category: FDA Approved
Reference Range: See laboratory report
Alternative Names: AFB PCR
Preferred Specimen(s): Unprocessed sputum

Processed sediment
Alternative Specimen(s): N/A
Minimum Volume: Unprocessed sputum - 1 mL

Processed sediment - 0.5 ml
Special Instructions: N/A
Reject Criteria: Inadequately labeled specimens

Leaking specimens

QNS (quantities not sufficient)

Specimens not meeting the Storage & Transport criteria upon receipt at laboratory
Setup Schedule: Testing is performed on an as-needed basis.
Turn around time (TAT): 2 business days from arrival at the lab.

Acceptable Specimens	Minimum Volume	Frozen Range -70°C - 0°C	Refrigerated Range 0.1°C - 8°C	Room Temperature Range 8.1°C - 30°C	Stability at 4°C - 25°C
		Specimen Storage			Specimen Transport
Unprocessed sputum	1 mL	Reject	Acceptable up to 7 days	Acceptable up to 35°C for 3 days	8 hours
Processed sediment	0.5 mL	Reject	Acceptable up to 7 days	Reject	8 hours