STEC Clearance Culture (Public Health Nursing use only)

Public Health Nursing use only - fee applies to Local Health Jurisdiction of submitting facility.

Testing for the presence of Escherichia coli (Shiga-toxin) DNA from stool samples.

Storage and Transport Criteria: Specimen Storage Specimen Transport

Acceptable Specimens

Minimum Volume

Frozen Range
-70°C - 0°C

Refrigerated Range
0.1°C - 8°C

Room Temperature Range
8.1°C - 30°C

Stability at
4°C - 25°C

stool in modified Cary Blair Enough stool added to Cary Blair transport media system for the volume to reach the 'fill line' of the transport vial. Reject Acceptable Acceptable 96 hours (in Cary Blair transport media system)
Includes: CHROMagar differentiation

STEC PCR
Methodology: Culture
Assay Category: LDT (Laboratory Developed Test)
Reference Range: See laboratory report
Alternative Names: Clearance
Preferred Specimen(s): Stool in modified Cary Blair
Alternative Specimen(s): N/A
Minimum Volume: Enough stool added to Cary Blair transport media system for the volume to reach the 'fill line' of the transport vial.
Special Instructions: Stool must be preserved in Cary Blair within 2 hours of collection.
Reject Criteria: Inadequately labeled specimens

Leaking specimens

QNS (quantities not sufficient)

Specimens not meeting the Storage & Transport criteria upon receipt at laboratory
Setup Schedule: Testing is performed on an as-needed basis.
Turn around time (TAT): 4 weeks from arrival at the lab

Acceptable Specimens	Minimum Volume	Frozen Range -70°C - 0°C	Refrigerated Range 0.1°C - 8°C	Room Temperature Range 8.1°C - 30°C	Stability at 4°C - 25°C
		Specimen Storage			Specimen Transport
stool in modified Cary Blair	Enough stool added to Cary Blair transport media system for the volume to reach the 'fill line' of the transport vial.	Reject	Acceptable	Acceptable	96 hours (in Cary Blair transport media system)