Norovirus detection, RT-PCR

The Norovirus RT-PCR Assay is designed to detect both norovirus genotype I (GI) and norovirus II (GII) strains.

Storage and Transport Criteria: Specimen Storage Specimen Transport

Acceptable Specimens

Minimum Volume

Frozen Range
-70°C - 0°C

Refrigerated Range
0.1°C - 8°C

Room Temperature Range
8.1°C - 30°C

Stability at
4°C - 25°C

Unpreserved stool 1 g or 1 mL Acceptable Acceptable Reject 8 hours
Includes: Test distinguishes norovirus genotype I and norovirus genotype II.
Methodology: RT-PCR
Assay Category: LDT (Laboratory Developed Test)
Reference Range: See laboratory report
Alternative Names: Human Calcivirus

Nororvirus RNA, RT-PCR

Norovirus I and II PCR

Norovirus RNA

Norwalk Virus
Preferred Specimen(s): Unpreserved stool
Alternative Specimen(s): N/A
Minimum Volume: 1 g or 1 mL
Special Instructions: N/A
Reject Criteria: Inadequately labeled specimens

Leaking specimens

QNS (quantities not sufficient)

Specimens not meeting the Storage & Transport criteria upon receipt at laboratory
Setup Schedule: Testing is performed on an as-needed basis.
Turn around time (TAT): 2 business days from arrival at the lab

Acceptable Specimens	Minimum Volume	Frozen Range -70°C - 0°C	Refrigerated Range 0.1°C - 8°C	Room Temperature Range 8.1°C - 30°C	Stability at 4°C - 25°C
		Specimen Storage			Specimen Transport
Unpreserved stool	1 g or 1 mL	Acceptable	Acceptable	Reject	8 hours