Lyme Enzyme Linked Immunosorbent Assay IgM/IgG

In this algorithm, serum is first tested in an immunoassay measuring combined IgG and IgM antibodies to specific borrelial proteins; if reactive, the sample is then tested in separate Borrelia burgdorferi IgG and IgM immunoassays. This algorithm may be able to assist in the identification of early Lyme disease within the first 30 days of infection.

Portal Ordering Code: LYME
CPT Code(s): 86618

Fee Schedule

Storage and Transport Criteria: Specimen Storage Specimen Transport

Acceptable Specimens

Minimum Volume

Frozen Range
-70°C - 0°C

Refrigerated Range
0.1°C - 8°C

Room Temperature Range
8.1°C - 30°C

Stability at
4°C - 25°C

Serum 1 mL Acceptable at -20°C or lower Acceptable Acceptable 8 hours
Includes: If Lyme Disease Antibody is Positive or Equivocal, then Lyme Disease Supplemental Antibodies (IgG, IgM), Immunoassay will be performed at an additional charge (CPT code(s): 86617(x2))
Methodology: ELISA
Assay Category: FDA Approved
Reference Range: See laboratory report
Alternative Names: MTTT-2

Borrelia VlsE1 pepC10

B. burgdorferi Ab

Lyme Early

Borrelia ELISA

B burgdorferi

Lyme Titer

Modified Two Tiered Test

Lyme, Serum

Borrelia burgdorferi

Lyme ELISA

Lyme Serology
Preferred Specimen(s): Serum
Alternative Specimen(s): N/A
Minimum Volume: 1 mL
Special Instructions: 1 mL of serum is sufficient volume to perform all tick-borne disease serology assays. Submission of multiple tubes of serum in not necessary.
Reject Criteria: Inadequately labeled specimens

Leaking specimens

QNS (quantities not sufficient)

Specimens not meeting the Storage & Transport criteria upon receipt at laboratory
Setup Schedule: Testing done once a week.
Turn around time (TAT): 7 business days from arrival at the lab.

Acceptable Specimens	Minimum Volume	Frozen Range -70°C - 0°C	Refrigerated Range 0.1°C - 8°C	Room Temperature Range 8.1°C - 30°C	Stability at 4°C - 25°C
		Specimen Storage			Specimen Transport
Serum	1 mL	Acceptable at -20°C or lower	Acceptable	Acceptable	8 hours